This page contains links to datasets produced within the project:

• Fibroblast and PC3 cell imaging data
  For both datasets cells were labeled with 3 different fluorophores: DAPI (4',6-diamidino-2-phenylindole) for the nuclei, while fibrillarin antibody (fibrillarin) and ethidium bromide (EtBr) were used for nucleoli staining. Original 3-channel volumes were aquired using laser scanning confocal microscope. They were then cut into 1024x1024xZ sub-volumes and channels were separated into c0, c1, and c2, correspondingly. Each link to data archive below represents one "run" of the microscope and contains original 1024x1024xZ TIFF sub-volumes in all 3 channels, as well as nuclear and nucleolar masks after segmentation and quality control. Each tarball also contains README file with detailed description of file structure inside.

  Sample preparation protocols: PDF

• Caco-2 cell imaging data
  Cells were labeled with 4 different fluorophores: DAPI (4',6-diamidino-2-phenylindole) for the nuclei, HES1 Alexa 647 (1:200; Abcam), NR3C1 Alexa 488 (1:150; Cell signaling) and OCLN Alexa 594 (1:600; Thermo Fisher). Original multichannel volumes were aquired using laser scanning confocal microscope. For protocol details see [4]. Images were saved in Analyze image format, in which each 3D image is represented by a pair of files: .img and .hdr.

Usage: You are granted a non-exclusive license to use these images for non-commercial, research purposes, with the following conditions:

1. you agree to include a reference to the corresponding paper when presenting or publishing your results,
2. you agree not to re-distribute these images without inclusion of this notice.

• Fibroblast
• PC3
• Caco2